These all-inclusive assay kits are ready to use in assay validation or screening. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionHere, we present FluoroBrite™ DMEM, a cell culture media designed specifically for live-cell imaging that, when excited at wavelengths commonly used for FITC/GFP (i. free tracer decreases, resulting in an. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesand emission wavelengths for SpectraMax M5 fluorescence microplate readers are as follows: excitation at 490 nm and emission at 525 nm, with 515-nm emission cutoff filter (note that these are different from the wavelengths recommended for filter-based readers in the Quant-iT PicoGreen product insert). The SpectraMax M5 is a microplate reader that can be used for various applications, such as ELISA, Western blot, or cell culture. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes 6. New drivers take 6 days to write, existing drivers take 2 days to convert to Windows 7/10 compatibility. 0 mL). Detection of pro-inflammatory factors. For very concentrated samples, use a minimum2. For SpectraMax L or LMax II, LMax II. Bookings are free, but you will need to use iLab to book. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. Plate chamber design of the SpectraMax category 1 microplate readers. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5 Multi-Mode Microplate Reader. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. In either case. 72 MB. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. 3. Cavicide (Warehouse, Cat # 79300360) 6. 306 plate air cal. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. . In this product video, see how the SpectraMax M5 quickly reads samples. MDS Analytical Technologies announced the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. The analysis capabilities ofThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. free shipping. 190-1000 nm, tunable in 1 nm increments. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax Spectrofluorometers. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Sale. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Table of Contents. Purchase Overlord with the required number of. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Other technologies such as imaging. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The chamber. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. The following document is intended to demonstrate setup of this. Step 1: Open the SoftMax Pro IMAP FP protocol. When the correct COM port is selected, the. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode We would like to show you a description here but the site won’t allow us. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity, permittingwidespreaduseincludingELISAs,proteinquantitation,endotoxinassays,and cytotoxicityassays. . The M5 can read volumes down to 2uL with the. These lab workhorses are used in drug discovery, research, bioassay validation, and. . The time course of the cleavage reaction was monitored by loss of FRET (excitation, 450 nm; emission, 655 nm) with 15 reads/well and 4–5-s read intervals on a SpectraMax M5 plate reader (Molecular Devices). VWR offers biophotometers for your lab's needs. Example: [Drawer]. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. EarlyTox Live Cell Assay (6. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. . . 304 signal level saturation. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 1. 315 can't find zero order. Sale. Catalog Number: M5. ; The top 3 importers of Spectramax are India with 365. Samples were measured together with a corresponding reference solvent contained in a. 305 reference level saturation. -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. When a feature or capability applies to only certain readers, this exception is noted. 3: The cuvette chamber. . ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e• SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. Optimal instrument settings are the same for all three readers. 4. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 313 reference gain check fail. Description 1. ® reader to the computer. Multimode Reader Comparison Chart. Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit The Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. Example: [Drawer]. 6: bioNova científica, s. SpectraMax® Microplate Readers and SoftMax® Pro Software. Bookings are free, but you will need to use iLab to book. Typical LLDs were approximately 1–2 fg/well in the Analyst GT and 2–3 fg/well in the SpectraMax M5. . SpectraMax Quant dsDNA Assay Kits. 5. Add to Cart. . $4,499. Set the excitation at 531 nm and emission at 595 nm. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. 0 (GraphPad Software, La Jolla, CA, USA) and SPSS version 23. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. . 1. If you do not have an iLab account you must first make one: SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. Unit comes with software and computer. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Step 2. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. D 1 1. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. The absorbance and photo-luminescence was determined on a SpectraMax M5 plate reader via quartz cuvette using 1 × 10 10 particles per ml or lower and extrapolated when an optical density of 1 was. . 303 unable to cal dark current. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. Step 4: Incubate at room temperature for 1 hour, protected from light. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. 2. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Turn on the microplate reader. spr SpectraMax iD5 SpectraTest ABS2 v1. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. . 多功能酶标仪SpectraMax M5e. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. ) Open SoftMax Pro, set Preference to SpectraMax L, and try the different available COM ports to find the one for the SpectraMax L. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. 1 or greater versions. The dual monochromator optics allow. Spectranet customer portalMice were fasted overnight and orally administered FITC-dextran (600 mg/kg). 95. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. I have been using the SpectraMax M5 since 2011. Open the Softmax Pro software and select the FP option. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. This application note describes how to. 0 out of 5 $ 17,999 $ 16,995. HEALTH AND SAFETY CONSIDERAIONS StakMax Handling Stacker Base Unit. 0 out of 5 $ 15,999. 3: The cuvette chamber. Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. e. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Spectranet customer portal. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. Read fluorescence polarization at room temperature (RT, 19–25°C). Description 1. 312 gain calibration failed. 19. Add to Cart. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. ItSpectraMax® M5多功能读板机为使用者提供了除化学发光以外的其他检测方法，例如：光吸收，荧光强度，荧光偏振和时间分辨荧光。 SpectraMax® L和SpectraMax® M5酶标仪均用SoftMax® Pro软件进行操控和设置，该软件含有CellTiter-Glo检测模板和其他120多个分析模板，这些. Cables. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Molecular Devices SpectraMax M5 Multimode Microplate Reader. . Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. . View the Molecular Devices websiteSpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. • Tel. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. 40266 Email: drugdiscoverytech@lifetech. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. 3: The cuvette chamber. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Maximizes fluorescent signal from a wide range of cell types by reducing cell wash artifacts. The test solution was by diluting FCV to PBS/ Glycerol solutions (5 μM, 1. 1) SpectraMax Glo Steady Luc Reporter Assay (6. The following document is. 2. The dynamic range of the assay was approximately 5 orders of magnitude. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the. The SpectraMax M5/M5e & FlexStation 3 instruments use SoftMaxPro® Data Acquisition & Analysis Software. 2. The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 1. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. There were 12 replicate wells per dilution in both 96-well and 384- well plates. . For example, microplates containing different samp les can be read using the same or Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. SpectraMax ABS Plus SpectraTest ABS2 v1. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. SpectraMax M5 device. When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. For example, microplates containing different samples can be read using the The interval between reads was set at 15 s or 60 s, respectively. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideIn this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. Microplate Reader Shelf. 1. If you do not have an iLab account you must first make one: Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. +44-118-944-8000. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Principle of the HTRF IP-One competitive binding assay. 2 mm long, 86. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. LUMINESCENCE The SpectraMax M2 and SpectraMax M2e are microplate spectrofluorometers with photomultiplier tube detection. After 4 h, the fluorescence intensity of serum FITC-dextran was obtained at 480 nm excitation wavelength and 520 nm emission wavelength using a Spectramax M5 microplate reader (Molecular Devices). SpectraMax DuoLuc Reporter Assay (SMP 6. Molecular Devices ®(MD) SpectraMax 340PC reader MD VersaMax™ Tunable Microplate readers MD SpectraMax® L Microplate Luminometers MD SpectraMax® M5 systems MD ®SpectraMax 96/384-well plate reader Gemini fluorescence plate reader Automation Hamilton Microlab® STAR™ liquid handling systemThe SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. ) Turn on the SpectraMax L and connect the computer to the SpectraMax L using the USB cable. . Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Highly selective for dsDNA over ssDNA or RNA. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. The cells were then washed and staining quantitation was performed using a fluorescent plate reader (SpectraMax M5 Molecular Devices, Sunnyvale, CA) at 492/520 excitation emission wavelengths. The following document is intended to demonstrate setup of this instrument. These robust readers have been placed in labs from Antarctica to the. ) Click the "OK" button on SoftMax Pro Setup dialog box . Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Customer. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Place the plate into a SpectraMax M5 e plate reader (Molecular Devices). It does not work as expected. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9THE SPECTRAMAX PLUS Two ways to correct pathlength The SPECTRAmax PLUS gives you two options when making pathlength-cor-rected measurements: 1) putting the aqueous solvent into a clean 1 cm reference cuvette in the instrument’s cuvette port and having the instrument base the calcu-lations on the A 1000 and A 900Molecular Devices社の製品: マイクロプレート リーダー用ソフト SoftMax® Proに関するすべての情報をご覧ください。価格、見積もり、お近くの販売店を知るにはメーカーまたは本社に直接お問い合わせくだ. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Instructions on the proper use of the SpectraMax M5 Plate Reader. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. 190 - 1100 nm. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 3. SpectraMax L Microplate Reader provides medium to high throughput. All reagents were brought to room temperature before use. Table of Contents. Reduces well-to-well variability, improving assay quality (Z' factor) and reliability (CV %) of high-throughput. For example, microplates containing different samples can be read using the312 gain calibration failed. SoftMax Pro software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. SpectraMax M5 and the Analyst GT. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. Cell viability was assessed by CellTiterGlo (Promega) reagent 96 hours after beginning treatment, and luminescence was measured on a Spectramax M5 spectrophotometer. 72 MB. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. For example, microplates containing different samp les can be read using the same orPlates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. 1) EarlyTox Glutathione Assay (6. Fix: While proper use of the Shake button is to hold the button down for the duration of the desired mixing time, repeated sequential clicking is now allowed. 3 or greater. Spectramax M5 plate reader (96/384 format) Multimode photometrics for endpoint or kinetic measurements. Keywords: spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software,. SoftMax Pro Software combines reader control and data analysis and provides sophisticated curve fitting and statistics for more in-depth bioassay analysis. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. NORTH AMERICA. spr SpectraMax Plus 384. The guide also covers the software features, the specifications, and the accessories of the systems. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Description 1. 4. Choose from a three- (M3), four- (M4), orThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. A wireless light sensor (701-999, PS-3212) was purchased from PASCO. Setup recommendations for SpectraMax® M5e. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. In addition, the SpectraMaxMolecular Devices. Select the fluorescence option and read the total fluorescence. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Molecular Devices SpectraMax PLUS 384 Spectrophotometer System-New ABs1 Performance Testing August 2021. HTRF-compatible SpectraMax Multi-Mode Readers. The following document is intended to demonstrate setup of this instrument. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. The plate reader can measure multiple readouts, including UV. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Fluorescence spectra and UV–vis spectra were recorded on a SpectraMax ® M5 multi-mode microplate reader (Molecular Devices). The following document is intended to demonstrate setup of this instrument. Published Dec 16, 2020 | Updated Dec 16, 2020. 01) between the GFP-transfected and mock-transfected cells at an initial seeding. The chamber contains. 304 signal level saturation. There were 12 replicate wells per dilution in both 96-well and 384- well plates. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Open the SoftwarePro® Data Acquisition & Analysis software,. View the Molecular Devices websiteSpectraMax® M5 multimode microplate reader from Molecular Devices to measure both reporters in a completely homogeneous manner, in the same well of a 96-well plate, without cell lysis. We would like to show you a description here but the site won’t allow us. For more sample throughput, the system canMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. 00 USD. 1. The chamber. Three kits covering a wide range of dsDNA concentrations. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Supplier: Molecular Devices. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. Fluorescent Polarization: Solid black plates, as above. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. 4. 5. EUROPE. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). ScanLater Western Blot Detection Cartridge. The chamber contains springs that position the cuvette in the proper alignment for a read. 0 out of 5 $ 15,999. The day after, a final concentration of 1 μmol/L ARS1620, 20 nmol/L SMARTpool siRNA or the combination of the 2 was added in 5 replicates (wells). This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. used. you can use a resource. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. The excitation and the emission. 3. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. 005) Photometric PrecisionThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. A preconfiguredAcme Revival: Long Live DevicesWe would like to show you a description here but the site won’t allow us. The SpectraMax M5 Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence intensity, glow luminescence, fluorescence polarization and time-resolved fluorescence (TRF). . 多功能酶标仪 FlexStation3 快速动力学操. Control Panel The control panel houses the instrument status LEDs and the microplate. HEALTH AND SAFETY CONSIDERAIONS The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. Choose from a three- (M3), four- (M4), or SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity,. STAKMAX. ORDER. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. These robust readers have been placed in labs from. Consistent results are obtained over time offering workflow flexibility. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. Offer Ends In: 18 Days 19: 38: 21. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. . SoftMax Pro. e. Table of Contents. Description. 316 grating motor driver faulty. Overall, SpectraMax M5 is a powerful lab tool that combines advanced detection capabilities, user-friendly software, and comprehensive accessory options. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeWe would like to show you a description here but the site won’t allow us. . The integration time suggested is 5-10 sec. The M5 can read volumes down to 2uL with the uMax Low.